casein kinase 2 ck2 substrate motif  (Cell Signaling Technology Inc)

Journal: Protein science : a publication of the Protein Society

Article Title: The molecular details of a novel phosphorylation-dependent interaction between MRN and the SOSS complex.

doi: 10.1002/pro.4782

Figure Lengend Snippet: FIGURE 2 INTS3 is a CK2 substrate. Immunoprecipitation experiment of endogenous INTS3 from lysates of U2OS cells either untreated or exposed to 6 Gy of gamma-irradiation (ionizing radiation; IR). Anti-INTS3 and anti-hSSB1 antibodies were used to assess immunoprecipitated endogenous INTS3 and hSSB1, respectively. CK2 phosphorylation was assessed by probing with an antibody specific for CK2 phosphorylation motifs within substrates. Representative of three independent repeats.

Article Snippet: Antibodies against the Casein Kinase 2 (CK2) substrate motif (#8738) were purchased from Cell Signaling Technology (Genesearch).

Techniques: Immunoprecipitation, Irradiation, Phospho-proteomics

Journal: Protein science : a publication of the Protein Society

Article Title: The molecular details of a novel phosphorylation-dependent interaction between MRN and the SOSS complex.

doi: 10.1002/pro.4782

Figure Lengend Snippet: FIGURE 3 Alignment of known CK2-phosphorylation- dependent Nbs1 recognition sites. Sequence alignment of INTS3584–597 with other Nbs1 binding peptides from human MDC1, human CtIP, Schizosaccharomyces pombe Ctp1, and S. pombe Lif1. Sequence alignments were performed using the M-Coffee web server (Moretti et al., 2007). Light and dark gray areas indicate large similarities or sequence conservation, respectively, whereas light blue areas indicate conserved aspartate and glutamate residues.

Article Snippet: Antibodies against the Casein Kinase 2 (CK2) substrate motif (#8738) were purchased from Cell Signaling Technology (Genesearch).

Techniques: Phospho-proteomics, Sequencing, Binding Assay